In no particular order and also much more than 5. Hope you like long lists!:
Minthara Baenre (Baldur's Gate 3)
Ianthe & Coronabeth Tridentarius (The Locked Tomb)
Harrowhark Nonagesimus (The Locked Tomb)
Cunoesse (Disco Elysium)
Alice Liddell (American McGee's Alice/Alice Madness Returns)
Anna Angel (Pathologic)
Samarie (Fear and Hunger)
Ashley Graves (The Coffin of Andy and Leyley)
Mad Moxxi (Borderlands)
Junko Enoshima (Danganronpa)
Jinx (Arcane) (REAL Jinx not what S2 did to her)
Jeanette Voerman (Vampire the Masquerade: Bloodlines)
Genocider Syo (Danganronpa)
Mikan Tsumiki (Danganronpa)
Kuroko Koumori (Murcielago)
GLaDOS (Portal)